Conformational Change

• Confirm specificity
• Resolve binding
  mechanisms
• Measure birefringence and
  order in lipid bilayers

Specific to biochemical activity. Non specific binding does not induce a conformational response and monitoring the conformation activity relationship provides great insight into biochemical mechanisms. Conformational change can occur not only from binding but also from temperature change, pH, ionic strength, light stimulation, or a host of other environmental effects. The 4D Workstation can measure all of these without the need for any mass change associated with binding providing a comprehensive biophysical characterisation.

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Application notes:

• Measurement of Conformational Change in Prion Protein on Calcium Binding
  Measurement of Conformational Change in Prion Protein on Calcium Binding Introduction Metal ions, especially divalent metal cations, play an essential role in a wide range of biological processes.
• Real Time Measurement of Actin Polymerisation by DPI
  Real Time Measurement of Actin Polymerisation by Dual Polarisation Interferometry Introduction This application presents Dual Polarisation Interferometry (DPI) as an important tool for investigating and understanding actin polymerisation.
• Biotin - Streptavidin Interactions: Measuring Conformational Change and Differentiating between Specific and Non-Specific Binding
  Biotin - Streptavidin Interactions: Measuring Conformational Change and Differentiating between Specific and Non-Specific Binding Introduction Dual Polarisation Interferometry (DPI).